Resources
iEAR: inner Ear Antibody Resource
Searchable file with details on the antibodies the Pyott Lab has used for immunofluorescence in the inner ear (includes antibodies that do and do NOT work, published and unpublished). The latest version contains over 150 entries. [Excel File]
Protocol for applying RNAscope and immunofluorescence in the isolated organ of Corti. Additional protocols for quantitative image analysis. [STAR Protocols]
Protocol for isolating and immunofluorescently staining the isolated auditory and/or vestibular sensory epithelia [PDF]
Protocol for calculating the areas of immunopuncta (especially hair cells ribbons or efferent terminals) [PDF]
Protocol for analysis of confocal image stacks in Imaris [PDF]
Protocol for patch clamping inner hair cells in the intact organ of Corti from mouse or rat [PDF]